The functional assessment of autotransplanted splenic tissue by its capacity to remove oxidatively modified erythrocytes

HAKLAR G., Demirel M., Peker Ö., Eskitürk A., Işgör A., Söyletir G., ...Daha Fazla

Clinica Chimica Acta, cilt.258, sa.2, ss.201-208, 1997 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 258 Sayı: 2
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1016/s0009-8981(96)06455-8
  • Dergi Adı: Clinica Chimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.201-208
  • Anahtar Kelimeler: Carbonyl content, Heinz body, Splenic autotransplantation, Thiobarbituric acid reactive substances
  • İstanbul Kent Üniversitesi Adresli: Hayır

Özet

Free radicals and reactive oxygen species have been implicated in the pathogenesis of a variety of hematologic diseases and erythrocyte aging. Aged erythrocytes are removed from the circulation primarily by the spleen. In this study, we aimed to determine the functional effectiveness of autotransplanted splenic tissue by its capacity to remove oxidatively modified erythrocytes from the circulation. Our experimental model in rats include splenectomy with autotransplantation of 80% of the excised splenic tissue into the omental pouch. In this model, free radical damage was estimated by different parameters of lipid peroxidation such as carbonyl content and thiobarbituric acid reactive substances (TBARS), together with Heinz body formation. Our results have shown that splenic autotransplantation was effective in removing oxidatively modified, aged erythrocytes from the circulation.