Phytochemical Characterization and In Vitro Anti-Tyrosinase Activity of a Traditional Hypericum perforatum L. Olive Oil Macerate for Cosmetic Applications
Cosmetics, cilt.13, sa.4, 2026 (ESCI, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 13 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/cosmetics13040194
- Dergi Adı: Cosmetics
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Applied Science & Technology Source, Directory of Open Access Journals, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Anahtar Kelimeler: cosmetic application, Hypericum perforatum, molecular docking, naphthodianthrones, olive oil macerate, phytochemical characterization, skin permeability, tyrosinase inhibition
- İstanbul Kent Üniversitesi Adresli: Evet
Özet
Hypericum perforatum L. olive-oil macerate is a traditional topical remedy whose wound-healing efficacy is well documented, yet the phytochemical and mechanistic basis of its cosmetic-relevant activity remains poorly defined. This study provides an integrated experimental and in silico characterization of a traditionally prepared macerate of defined origin. The fatty acid composition, oxidative-stability indices, total phenolic and flavonoid contents, naphthodianthrone level, antioxidant capacity, and tyrosinase inhibition were determined, and the principal constituents were examined by molecular docking and in silico skin-permeation and toxicity profiling. The olive-oil matrix was oleic acid-dominated (73.9%) and oxidatively stable (iodine value 86.0; oxidizability index, COX, 1.84), while the macerate showed modest phenolic ((Formula presented.) g pyrocatechol equivalents, PCE/mg) and naphthodianthrone (0.044 mg/mL hypericin equivalents) contents. It showed limited antioxidant activity but moderate tyrosinase inhibition (38.75% at (Formula presented.) g/mL). Docking revealed that, despite high affinity scores, the naphthodianthrones lay 7–9 Å from the catalytic copper centre (an artefact of the scoring function), whereas fatty acids accessed the active site more closely, implicating the matrix in the observed activity. In silico profiling indicated that the photoactive naphthodianthrones are largely retained at the skin surface. These findings link this centuries-old preparation to a mechanistically informed understanding of its cosmetic potential.